Molecular Characterization and Expression Response of Ghrelin, GLP-1 and PYY to Fasting, Dietary Lipid, and Fatty Acids in Silver Pomfret (Pampus argenteus)

Ghrelin, glucagon-like peptide-1 (GLP-1), and peptide YY (PYY) are potent hormones mediating food intake according to the nutritional status in fish. However, limited information is available on these genes their expression response nutrition silver pomfret (Pampus argenteus). A comparison analysis revealed that ghrelin GLP-1 were relatively conserved marine PYYa PYYb shared a low identity clustered different PYY branches. pyya, pyyb mRNAs highly expressed brain, while glp-1 was gills liver. The mRNA high an hour post-feeding decreased after 3 72 h of fasting. increased 6 24 pyya fasting, remained stable during diet with 14% lipid promoted expressions glp-1, pyyb, but inhibited expression. intestine palmitic acid, oleic acid (OA), linoleic (LA), α-linolenic eicosapentaenoic (EPA), docosahexaenoic (DHA) incubation. OA pyya. downregulated by LA, DHA, EPA. These results suggest importance ghrelin, pyy coordinating dietary concentration, fatty acids pomfret.